Stay up to date with the latest research, tips, and trends in fitness, nutrition, and weight management.
India’s cervical and breast cancer screening rates remain abysmally low, with delayed HPV vaccination, weak referrals, and high costs driving late-stage diagnosis.
Measles deaths in Bangladesh rise to 311 as infections surge nationwide, raising urgent concerns over vaccination gaps and public health response.
A common recessive NDD caused by RNU2-2 mutations disrupts brain development and opens paths for diagnosis and therapy.
The present work aims at evaluating a novel therapeutic technique against clinical samples of Pseudomonas aeruginosa, a multiple drug resistant bacterium by integrating gold nanoparticles (AuNPs) with antisense oligonucleotide (ASO) (ASO-AuNPs) to target and silence the blaVIM and blaCTX-M genes. The objectives were to evaluate the ability of ASO-AuNPs to silence resistance genes and reduce their expression to combat antibiotic resistance. Seventy P. aeruginosa clinical isolates collected from Iraqi hospitals were isolated, identified and their antibiotic resistance profiles were assessed. The blaVIM and blaCTX-M genes were detected by PCR. These genes were engineered through the use of ASO-AuNPs. Resistance to carbapenem and cephalosporin and quantitative blaVIM and blaCTX-M gene expression in P. aeruginosa were further studied before and after silencing. The blaVIM gene was found in 79.2% of carbapenem-resistant samples, while blaCTX-M was identified in 72.7% of cephalosporin-resistant samples. A significant reduction in antibiotic resistance was achieved following ASO-AuNPs application. Moreover, the gene silencing technique significantly decreased the expression of blaVIM and blaCTX-M genes. The mean fold change of the CTX-M gene was 15.92 before and 1.36 after gene silencing, that of the blaVIM gene was 0.63 and 0.51, respectively. These findings provide a proof-of-concept for the use of ASO-functionalized gold nanoparticles as a potential strategy for targeting antibiotic resistance genes in P. aeruginosa, with potential applications in managing resistant infections in healthcare settings.
Researchers from the College of Liberal Arts and Sciences examined differences in childhood adversity between Black men and women and its impact on heart di ...
A cruise ship is marooned in the Atlantic Ocean with seven confirmed or suspected hantavirus cases on board,...
While high income countries may eliminate cervical cancer by 2050, women in other regions are likely to remain at risk, found a recent Lancet study
Nitroreductases (NRs) can reduce nitroaromatic compounds, which are toxic, mutagenic or carcinogenic, to nitrite, amino group or hydroxylamine groups. We have utilised a cloned nitroreductase from a moderate thermophilic Bacillus species in E. coli BL21. Purification of the enzyme, followed by electrophoresis and western blotting, revealed approximate molecular weight of 30 kDa. The optimum temperature and pH of the enzyme was found as 40 °C and 8.0, respectively. Conversion of CB1954 to the metabolic products and nitrofurazone reduction by enzyme in the presence NADPH as co-factor was studied. Km, kcat and kcat/Km values were determined as 42.5 (µM), 5.07 (s− 1) and 0.1194 (s− 1.µM− 1), respectively from the data using CB1954 as substrate and NADPH as cofactor. Meanwhile, the values obtained for nitrofurazone substrate were 52.7 (µM), 0.626 (s− 1) and 0.0119 (s− 1.µM− 1), respectively. The enzymatic reduction of CB1954 analyzed by LC–ESI–MS gave prominent molecular ions at the expected m/z values of CB1954 ([M + H]⁺: m/z 253), primary hydroxylamine intermediates ([M + H]⁺: m/z 239), and amino end-products ([M + H]⁺: m/z 223). The purified enzyme was also found to biotransform TNT to the product 2-amino 4,6-DNT. In addition, a proof-of-concept electrochemical detection platform based on immobilized nitroreductase produced a measurable cathodic response toward nitrofurazone, demonstrating the enzyme’s electrochemical applicability. ANOVA identified significant differences in activity levels among multiple groups, while regression analysis enabled prediction of enzyme responses and characterization of the underlying kinetic patterns in statistical analysis.
From childhood to old age, a urologist shares how often it's 'normal' to pee each day during every different stage of your life.
WHO reiterated that the risk to the wider public however, a limited spread among close contacts has been observed in some previous outbreaks.